In this study, we found that overexpression of miR-27b-3p not only inhibited the apoptosis but also simultaneously suppressed the EMT in TGF-β1-stimulated HK-2 cells, indicating that both apoptosis and EMT are involved in miR-27b-3p-mediated regulation in renal fibrosis. The gene discussed is TGFB1; the disease is renal fibrosis.