In this article, we verify the function of PKCα and find out the signalling pathways and related factors that may affect it through chip analysis, and assume their relevance, we finally through a series of experiments to verify the PKCa through regulating NF‐kB signalling pathway, in turn, affect the five key factor, ultimately affect the function of bladder cancer cell biology. This evidence concerns the gene PRKCA and urinary bladder cancer.