Strikingly, our FACS results revealed that there was an almost complete loss of chemotaxis by CD11b+Gr-1+ MDSCs in AMD3100-treated tumours (DMSO group: U87-sh-NC vs. U87-sh-554, p < 0.01; U87-sh-NC vs. U87-sh-1068, p < 0.01; AMD3100 group: U87-sh-NC vs. U87-sh-554 vs. U87-sh-1068, n.s. non-significant difference) (Fig. 5D). This evidence concerns the gene ITGAM and neoplasm.