Significant gender differences were observed due to the majority of the HNSCC patients being male (74% male vs 26% female), however a significantly higher percentage of females were observed in the high TLR8 expression patient cohort (39.6%) compared to low TLR8 expression (18%), while the percentage of males was significantly higher in the low TLR8 expression group (81.7%) compared to high TLR8 (60.4%) (Supplemental Table 1). The gene discussed is TLR8; the disease is head and neck squamous cell carcinoma.