To restore the differentiated cancer cells into CICs, expression vectors expressing c-Myc, TWIST1, OCT4, and SOX2 (CTOS) were separately transfected into v6 Mu1 SW948-FR CICs with or without a v6-rescue plasmid to express these genes and proteins (shown in Figure 4I,M). Here, POU5F1 is linked to cancer.