We analyzed the transcript level of randomly selected DEGs that presented up-regulation or down-regulation of the calcium signaling pathway (HTR2C, SLC8A3, BDKRB1, ATP2A2, and ADRB1), CM pathways (IGF1, ADRB1, and ACTC1), cytokine-cytokine receptor interaction (IL6), extracellular matrix (ECM) receptor interaction (ITGA8, and TNC), focal adhesion (COL1A1, and TNC), cardiac fibroblast markers (ACTA2, COL1A1, and VIM), and cardiac hypertrophy markers (NPPA, and NPPB) using qPCR. Here, ACTC1 is linked to cardiac hypertrophy.