In H508 colon cancer cells, inhibiting Src attenuated ACh- and EGF-induced ERK1/2 phosphorylation (activation), identifying Src as a key link between M3R-induced transactivation of EGFR and the subsequent downstream activation of MAPK (ERK1/2) in CRC [66]. This evidence concerns the gene MAPK3 and malignant colon neoplasm.