We found negligible expression of the pluripotency factors OCT3/4 and SOX2 in pYSTs, 1411H and GCT27 compared withthe EC / seminoma cell lines, while the proposed aYST key factors FOXA2, SOX17, GPC3, DUSP4, AFP, GATA6, APOA1 and FGB were highly expressed in pYSTs (Figure 2H). The gene discussed is APOA1; the disease is seminoma.