We found negligible expression of the pluripotency factors OCT3/4 and SOX2 in pYSTs, 1411H and GCT27 compared withthe EC / seminoma cell lines, while the proposed aYST key factors FOXA2, SOX17, GPC3, DUSP4, AFP, GATA6, APOA1 and FGB were highly expressed in pYSTs (Figure 2H). This evidence concerns the gene AFP and seminoma.