Whilst TNF-α induced a significant 2.6-fold increase in SERPINE1 mRNA in ND-DF, there was no effect of TNF-α in T2DM-DF (P = 0.0035 for effect of TNF-α, two-way ANOVA, n = 4; Fig. 5i). This evidence concerns the gene TNF and type 2 diabetes mellitus.