Three days after withdrawal of TNF-α, there was no significant difference between the senescence rates of ND-DF and T2DM-DF (1.78 ± 0.8 vs 0.69 ± 0.2 A.U., P = 0.1832, two-way ANOVA, n = 4). The gene discussed is TNF; the disease is type 2 diabetes mellitus.