We generated single-cell clonal populations (clone B11 and E12) of the T-ALL cells dually stably transfected with PGK-RLuc and Tet-FLuc (4188-PGK-RLuc/Tet-FLuc) to enable simultaneous, sensitive monitoring of all T-ALL cells (RLuc) and the MYC-expressing subset (FLuc). The gene discussed is MYC; the disease is acute lymphoblastic leukemia.