Cells grown in 2D on plastic can show different metabolic requirements compared to cells grown under more physiologically relevant conditions, and so we examined the effect of serine and glycine depletion and PH755 treatment on intestinal tumour organoids derived from Vil1-creER;Apcfl/fl (Apc) or Vil1-creER;Apcfl/fl;KrasG12D/+ (Apc Kras) mice (Fig. 2e and Supplementary Figure 2a). This evidence concerns the gene VIL1 and neoplasm.