NES and neoplasm: Examples include the control of the key γ34.5 virulence gene by a hybrid nestin enhancer-HSP68 minimal promoter which ensures expression of the HSV genome specifically in the nestin-positive glioblastoma cells [40] and the insertion of miRNA target sequences specific for selected tissues (e.g., brain, heart, or liver) to avoid off-tumor expression of key herpesviral proteins like ICP4 (infected cell protein 4), ICP27, UL8, and γ34.5 [20].