Similarly, we observed a 36% reduction in the number of TRAP-positive, multinucleated (≥3 nuclei) osteoclasts produced in the presence of MCF-7 breast cancer cells plus EO-MCF-7 cells compared to TRAP-positive, multinucleated (≥3 nuclei) osteoclasts produced in the presence of MCF-7 breast cancer cells plus naïve osteoblasts (Figure 4i). The gene discussed is ACP5; the disease is breast cancer.