Only one signaling pathway, axon guidance, was closely linked with highly expressed proteins including TUBA1C, TUBA3C, TUBB4B, LAMC1, MMP9, GPC1, MYH9, MYL6, A0A087WVQ6, HSPA8, RPLP2, RPS27A, TUBA3E, ACTB, HSP90AA1, TUBB8, HSP90AB1, TUBB3, TUBA4A, MYL12A, TUBA1B, PSMB3, KRT10, KRT1, KRT9, and ACTB in spermatozoa from infertile patients (Table 4). The gene discussed is ACTB; the disease is Infertility.