Although baseline random luteinizing hormone (LH), GnRH-stimulated follicle-stimulating hormone (FSH), the LH to FSH ratio, levels of testosterone, estradiol, and other hormones, and hormonal binding protein profile analysis can be used in the evaluation of precocious puberty, the gold-standard for biochemical diagnosis involves the assessment of LH after stimulation with exogenous LH-releasing hormone (LHRH), GnRH, or GnRH agonists [2,6]. This evidence concerns the gene PLOD1 and precocious puberty.