To examine the importance of MED13 in modulation of alkylation response beyond HAP1 cells, we next inactivated MED13 by CRISPR–Cas9 in glioblastoma G144 cells and generated two independent MED13 KO cell lines (MED13 KO cl.5 and cl.12) (Supplementary Figure S2D). This evidence concerns the gene MED13 and glioblastoma.