Further, we found that FAM225A promoted ESCC invasion and overexpression of miR-197-5p dampened cell invasion of KYSE30 or KYSE510 cells, whereas overexpression FAM225A together with miR-197-5p showed the similar invasive ability of ESCC cells with control group (Figure 6C). Here, FAM225A is linked to esophageal squamous cell carcinoma.