In fact, in vitro data demonstrated that the increment in LD amount, as well as in the expression of genes involved with its synthesis, PLIN2 and FASN18, was triggered exclusively by the exposure of the esophageal epithelium to the risk factors associated with EAC development, once no association between ESCC-associated risk factors and LD generation was observed. The gene discussed is PLIN2; the disease is esophageal squamous cell carcinoma.