The following 3 fluorescent signals were observed: (1) Hoechst (blue) is a nuclear stain used to identify the WBC position; (2) anti-CD45+ (red) binds to a CD45+ molecule, which is a general protein expressed in WBCs; and (3) anti-STAU2 (green) is an antibody that binds to a candidate protein marker for breast cancer screening. Here, PTPRC is linked to breast cancer.