Genes involved in appressorium and penetration peg functionality were upregulated in all three setups, including WISH (Water wettability, Infection, Surface sensing and Hyper-conidiation) G-protein coupled receptor protein, whose KO renders the fungus unable to develop appressoria and establishes the infection on intact rice leaves [54] and superoxide-generating NADPH oxidases NOX1 (MGG_00750) and NoxD (MGG_09956). This evidence concerns the gene NOX1 and infection.