In the next step, LY294002 and HAA2020 were tested alone and in combination with 5FU to investigate their possible chemosensitizing effect on HT29, HT29-5FU, HCT116, HCT116-5FU, MRC5, and HUVEC cell lines as in vitro model, and to test their effect on the important kinase pathways resulting from the Saudi CRC samples, the ABC transporters, in addition to their effect on cell cycle and apoptosis. Here, ABCG2 is linked to colorectal carcinoma.