Our previous study found that CD133−/EpCAM− Huh-7 cells, when passing through transwell selection, formed a unique subpopulation, Huh-7-trans, and had extremely high levels of MMP expression compared with their native Huh-7 hepatoma counterpart cells, and at the same time, the GLI1/2 expression levels were accordingly increased. This evidence concerns the gene EPCAM and hepatocellular carcinoma.