Moreover, via subgroup analysis, we found that the higher expression of ZIC2 mRNA in CD4+ T cells was associated with a better 5-year survival prognosis compared with the lower expression, Nevertheless, this association needs to be further validated in the real world setting in large-scale clinical studies (Figure 7); however, a higher expression of ZIC2 mRNA was not associated with any significant differences in the 1-, 3-, and 5-year survival rates in B cells, CD8 + T cells, macrophages, neutrophils, or dendritic cells of patients with liver cancer (P>0.001). Here, CD8A is linked to liver cancer.