The predictive value of CSF Nf-L levels (AUC = 0.87) regarding ALS diagnosis is increased when combined with CSF ICAM-1 and serum IFN-γ levels (AUC = 0.99) using multiple regression parameters (Fig. 5b).A cutoff value of 0.49 for the fitted values discriminate ALS from IPN patients with a specificity of 100% (78.20–100%) and a sensitivity of 85.71% (57.19–98.22%) with an AUC of 0.99 ± 0.01. The gene discussed is ICAM1; the disease is bile duct papillary neoplasm.