Specificity and sensitivity regarding ALS diagnosis of serum Nf-L concentration alone (AUC = 0.84; sensitivity = 90.70% (77.86–97.41%); specificity = 74.58% (61.56–85.02%); LR = 8.017) or a model including 3 diagnosis biomarkers (serum Nf-L, serum VCAM-1 and serum IFN-γ) were equivalent. Here, VCAM1 is linked to amyotrophic lateral sclerosis.