To test how EGR1 and DDIT3 affect each other, shRNAs that target the 3′-UTR regions of EGR1 or DDIT3 were transfected into HCC-15H cells using lentiviral vectors, followed by gene-rescue by transfection of an EGR1 construct (Lv. Here, EGR1 is linked to hepatocellular carcinoma.