To determine the immune phenotypes within the tumor cells, CIBERSORT analysis25 of the RNA-seq data was performed and it revealed a higher abundance of tumor resident resting mast cells (Fig. 4a and Supplementary Fig. 10a) and increased CXCL10 expression (Fig. 4b) in multiple tumor samples obtained after anti-PD-1 treatment indicative of the possible role of chemokine in the mobilization of these cells. This evidence concerns the gene CXCL10 and neoplasm.