To identify biological pathways associated with KDM5A expression in osteosarcoma, we performed gene set enrichment analysis (GSEA) on the transcriptome dataset and observed that 50 gene sets were different between 143B and KDM5A-KO cells, and 13 sets were enriched in 143B cells (FDR < 0.25, p < 0.05) (Fig. S3). The gene discussed is KDM5A; the disease is osteosarcoma.