We previously showed that CRC development is sustained by cancer stem cells (CSCs), whose dissemination is responsible for CRC metastasisation,33 suggesting that targeting disseminated CR-CSCs may prevent tumour relapse and increase survival of patients with CRC.31 Thus, we investigated the ability of PI3K inhibitors to target disseminated sphere cells in the liver before they were able to make metastases in a model of adjuvant treatment. This evidence concerns the gene PIK3CA and cancer.