As we have proved that activation of autophagy had an influence on CDCA4-regulated EMT, migration and invasion in NSCLC, the flipside of autophagy was also investigated using 3-methyladenine (3-MA), which is a class III phosphatidylinositol 3-kinase (Ptdlns3K) inhibitor, to treat both A549 and H1299 cells. This evidence concerns the gene CDCA4 and non-small cell lung carcinoma.