After a brief reminder of important insights on the structure and function of myosins of interest, this review aims at summarizing how some bacterial pathogens associated with important human diseases, such as EPEC, Neisseria, Salmonella, Shigella, Listeria, and Chlamydia, target host cell myosins to adhere to the host cell, to enter into the host cell, to evade autophagy/inflammasome degradation, to exit from the host cell, or to evade phagocytosis, ultimately promoting successful infection. The gene discussed is MYH14; the disease is infection.