Individually, all but one specimen demonstrated reproducible (Table S3) and clonal SB insertions sites, as evidenced by a range of low to very high read-depth-enriched TA-dinucleotide sites within the coding region of many known tumor suppressor genes such as Apc, Brd4, Cul3, Cux1, Gnaq, Nf1, Pten, Rasa1, Rnf43, and Wac (Figure 5 and Tables S14 and S15). The gene discussed is RNF43; the disease is neoplasm.