SIRT1 also interacts with NF-κB’s transcriptional corepressor- transducin-like enhancer of split-1 (TLE-1) and deacetylates lysine 310 on p65 protein leading to diminution of NF-κB activity [86,87] SIRT3—a transcriptional target of NF-κB, was shown to regulate inhibitory effect of metformin on NF-κB activity in differentiated L6 skeletal muscle cells with induced insulin resistance [88]. The gene discussed is NFKB1; the disease is Insulin resistance.