This is in contrast to the nitrogen at the 1-position, which appears to be crucial for the activity since its absence resulted in the inactive analogue 24 while the cinnoline 29 and quinoxaline 34 bearing Spautin-1 analogues showed some activity towards EGFR mutant NSCLC cells; however, they were less active compared to Spautin-1. This evidence concerns the gene EGFR and non-small cell lung carcinoma.