To investigate the role of JMJD2D in liver LCSCs, we enriched the LCSCs by inducing hepatoma spheroid formation from human liver cancer cell lines HepG2 and Huh-7 as well as mouse liver cancer cell line Hepa1-6 and then measured the protein and mRNA expression of JMJD2D. Here, KDM4D is linked to hepatocellular carcinoma.