For this validation, we selected 12 genes based upon their known involvement in preeclampsia and/or their very high splicing index differences between healthy and preeclamptic placentas: FLT1, CLDN1, LEP, FSTL3, TXK, CAP2, CA10, TNFRSF1B, ACOXL, TIE1, LAGLS14, CPXM2. The gene discussed is TIE1; the disease is preeclampsia.