AKT1 and breast cancer: For example, tumour necrosis factor receptor-associated factor 6 (TRAF6) is implicated in IGF-1-mediated ubiquitylation of Akt and the SCFSkp2 complex is important for EGF-dependent Akt ubiquitylation in breast cancer cell lines and mouse embryonic fibroblasts (MEFs) [38, 39].