This probe allowed us to analyze mTORC1 activity in GFP-expressing cells and measured its response to knocking out Raptor. We transduced mCherry-TOSI or control mCherry into CreERT-Raptorfl/fl AML cells and induced Raptor knock-out by adding hydroxytamoxifen (HTM). This evidence concerns the gene RPTOR and acute myeloid leukemia.