At last, we examined the relative expression of KLF6 in normal chondrocytes and several available chondrosarcoma cell lines by qRT-PCR; consistently, KLF6 expression was reduced in all the chondrosarcoma cell lines, and SW1353 and HCS2/8 cells showed the least expression of KLF6 (Fig. 5D). This evidence concerns the gene KLF6 and chondrosarcoma.