The top 10 genes frequently observed in cancer patients were ADCY9, RAB3GAP2, AURKAPS1, EYS, SHC2, DPP6, FREM2, ESR1, TBC1D22A, and ACTN2. The ten genes frequently observed in non-cancer subjects were SNTG2, LHFPL3, DACT2, NKAIN2, KALRN, ABR, LMNTD1, PLEKHA7, DOC2B, and ADPRHL1 (Supplementary Table 3). The gene discussed is ESR1; the disease is cancer.