Here we present the results of the expression analysis performed for genes from the MYC (MYCC, MYCN, MYCL) and E2F (E2F1, E2F2, E2F3) families and miRNAs from miR-17-92, miR-106b-25 and miR-106a-363 clusters in three types of pediatric brain tumors showing different histology and grade: medulloblastoma (WHO grade 4), ependymoma (WHO grade 2) and pilocytic astrocytoma (WHO grade 1). This evidence concerns the gene MYC and brain neoplasm.