PPARGC1B and Hyperglycemia: However, incubating C2C12 myoblasts, previously exposed to 3 days of hyperglycemia, with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) 0.50 mM for 24 h had no effect on miR-378a-3p and Ppargc1b expressions (Figure 1D,E).