Results from the immunofluorescence indicated that cytochrome c was located and accumulated in the nuclear, thus proposing that combination of HNHA, sorafenib, and radiation caused apoptosis via a cytochrome-c-dependent pathway in undifferentiated thyroid cancer cell lines, YUMC-A1 (Figure 5A,C) and YUMC-A2 (Figure 5B,D). The gene discussed is CYCS; the disease is thyroid cancer.