Caspase-1 activity (“active caspase-1”) was detected in situ on unfixed frozen sections of the tumor in all cases of CRC (n = 96) and in the paired normal colonic mucosa at distance from the tumor as a control (n = 10), using the fluorescent inhibitor probe FAM-YVAD-FMK (FLICA (Fluorochrome Inhibitor of Caspases) assay) that binds to the active form of caspase-1. The gene discussed is CASP1; the disease is colorectal carcinoma.