Then, we performed a multiplex bead-based assay (13-plex) for simultaneous detection of Th1/Tc1 cytokines and mediators, as well as Th17 and anti-inflammatory cytokines in the culture supernatants of 62 CRCs for which sufficient amounts of remaining supernatant were available prior the assay (n = 26 IFNγ− CRC and n = 36 IFNγ+ CRC). Here, NELFCD is linked to colorectal carcinoma.