To obtain a more quantitative assessment of aCasp1+ cells in the tumor microenvironment, we set up a multicolor flow cytometry panel combining a viability marker, caspase-1 FLICA assay, Epcam (for tumor cells), and CD11b (for myeloid cells) on cell suspensions obtained just after mechanical dissociation of fresh tumor fragments ex vivo (n = 10). Here, CASP1 is linked to neoplasm.