We designed an experimental approach on fresh tissues allowing us to assess caspase-1 activity, in situ and by flow cytometry using the FLICA assay, in relation with inflammasome-dependent cytokines (IL-18, IFNγ) secreted in the supernatants of ex vivo explant cultures of the tumor and with TIL density/phenotype. The gene discussed is IFNG; the disease is neoplasm.