CASP1 and neoplasm: In order to assess whether the absence of active caspase-1 in some CRCs could be associated with changes in caspase-1 mRNA levels as previously reported [27], we assessed the relative expression of caspase-1 mRNA by qRT-PCR, in the tumor and paired normal colon in the two subgroups, aCasp1+ (n = 8) and aCasp1− (n = 8).