To allow the evaluation of HEV remodeling in formalin-fixed paraffin-embedded (FFPE) biobank samples, we established a multicolor antibody panel for simultaneous evaluation of HEVs (marked by expression of the vascular addressin PNAd and the broadly expressed tight junction protein Claudin-5), FRCs (marked by expression of the chemokine CCL21), and tumor cells (marked by cytokeratin). Here, CLDN5 is linked to neoplasm.