In addition, in LNCaP prostate cancer cells, hydroxytyrosol inhibited cell proliferation in a concentration dependent manner (IC50 = 176 μM 48 h post treatment) through the suppression of Akt/STAT3 phosphorylation and by the cytoplasmic retention of NF-κB [37]. This evidence concerns the gene NFKB1 and Familial prostate cancer.