ACE and obesity due to melanocortin 4 receptor deficiency: Enzyme inhibitory assays were performed using lipase as the key enzyme to control obesity, α-amylase and α-glucosidase to test for diabetes, angiotensin-converting enzyme (ACE) to control hypertension, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-secretase (BACE-1) to control Alzheimer’s disease (AD).