High TSKU expression was strongly associated with poor overall survival of patients with lung cancer by multivariate Cox regression analysis, with HROS of 4.700 (95 % CI 2.360–9.360, P =1.10E-05) and HRRFS of 3.400 (95 % CI 2.030–5.810, P =4.00E-06), respectively. This evidence concerns the gene TSKU and lung cancer.