Among these candidate neoantigen genes, NEB, DNAH2, ABCA12, CENPF, and CELSR1 were newly identified as COAD biomarkers, while DNAH5, COL7A1, COL27A1, RP1L1, and ROBO2 had mutational profiles specific to COAD, compared to other solid tumors. The gene discussed is COL27A1; the disease is colon adenocarcinoma.