CCN2 and thalassemia: In addition, no appreciable CCN2 was detected in normal megakaryocytes in vivo (Astrom et al. 2015; Cicha et al. 2004; Sumiyoshi et al. 2010), although immunohistochemical staining indicated strong expression of CCN2 in megakaryocytes of primary myelofibrosis and in a subpopulation of megakaryocytes in patients with X-linked thrombocytopenia with thalassemia (Astrom et al. 2015).