To further address the role of Prune-1 in M2 polarization of macrophages, we used an anti-Prune-1 molecule (AA7.1) that has been previously shown to enhance Prune-1 degradation and to impair TGF-β signaling in metastatic medulloblastoma (Ferrucci et al., 2018). The gene discussed is TGFB1; the disease is medulloblastoma.